La RMN en el estudio del metaboloma humano: Definiciones y aspectos prácticos

Autores/as

Alma Leticia Saucedo; Eduardo Antonio Zul Hernández, Departamento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo Leó, México.; Aylín Irasema García Ortiz, Departamento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo Leó, México.; Juan Ricardo Lucio Gutiérrez, Departamento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, México.; Noemí Herminia Waksman Minsky, Departamento de Química Analítica, Facultad de Medicina, Universidad Autónoma de Nuevo León, México.

Palabras clave:

metabolómica, resonancia magnética nuclear, identificación de metabolitos, RMN cuantitativa, PCA, PLS-DA

Sinopsis

El metaboloma humano es una vasta colección de moléculas de bajo peso molecular como lípidos, péptidos, ácidos orgánicos, carbohidratos, aminoácidos y nucleótidos que actúan como sustratos o productos del metabolismo y que están relacionadas con funciones celulares esenciales como, nutrición, crecimiento y reproducción, diferenciación, señalización y apoptosis. El análisis integral del metaboloma es el tema central del enfoque de la metabolómica, y desde una orientación aplicada a las ciencias biomédicas y la investigación clínica, constituye una herramienta muy valiosa para conocer de forma global los procesos patológicos y conducir al descubrimiento de biomarcadores de diagnóstico, progresión y pronóstico de evolución. La RMN es una herramienta analítica que destaca por su robustez y reproducibilidad, ya que permite la detección, identificación y cuantificación de múltiples matabolitos de forma simultánea. Esto hace que sea uno de los métodos más empleados en los estudios de metabolómica. Los datos obtenidos con RMN son posteriormente sometidos a análisis estadístico multivariable con la finalidad de extraer la información diferencial entre los grupos de estudio a partir de su metaboloma, y conducir a una descripción biológica de los procesos relacionados con patologías e identificar biomarcadores, evaluar cambios asociados a la nutrición o variaciones ambientales, entre otras posibilidades.

Capítulos

  • La RMN en el estudio del metaboloma humano: Definiciones y aspectos prácticos
    Eduardo Antonio Zul Hernández, Aylín Irasema García Ortiz, Juan Ricardo Lucio Gutiérrez, Noemí Herminia Waksman Minsky, Alma Leticia Saucedo

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Publicado

April 15, 2022
Derechos de autor 2022 Imprenta de libros de la Universidad Tecnológica de la Mixteca